LOCUS       ISCR2-floR_unit         4290 bp    DNA     linear       20-NOV-2020
DEFINITION  Providencia rettgeri.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Providencia rettgeri
  ORGANISM  Providencia rettgeri
            Unclassified.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISCR2-floR_unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Providencia rettgeri"
                     /mol_type="genomic DNA"
     mobile_element  1..4290
                     /gene="ISCR2-floR unit"
                     /product="Putative resistance unit: ISCR2-floR unit"
     mobile_element  complement(1..1844)
                     /gene="ISCR2"
                     /product="Insertion sequence: ISCR2"
     misc_recomb     1..16
                     /gene="terIS"
                     /product="ISCR2 terIS"
     CDS             118..1611
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR2 transposase"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     CDS             1642..2526
                     /gene="virD2"
                     /codon_start=1
                     /transl_table=11
                     /product="Type IV secretory pathway, VirD2 components
                     (relaxase)"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRR
                     GVERVNCGPKLLDLGEKGVYSSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVERIDAD
                     QWRIPNDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGETADLA
                     PTGFGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSDMALS
                     KGLPFRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGREVMGI
                     VQGGSVSWQLGRQRGLER"
     misc_recomb     1826..1844
                     /gene="oriIS"
                     /product="ISCR2 oriIS"
     CDS             2743..3957
                     /gene="floR"
                     /codon_start=1
                     /transl_table=11
                     /product="Chloramphenicol/florfenicol resistance, MFS
                     efflux pump"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPDIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSMLAFVPALGPIAGT
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTYFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNGDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     CDS             3985..4290
                     /gene="lysR"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
BASE COUNT      818 a   1236 c   1328 g    908 t
ORIGIN
        1 gagtgacggg cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa
       61 gaactttccg ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg
      121 cctcatgtgg cggccaggac ggccagccgg gatcgggata ctggtcgtta ccagagccac
      181 cgacccgagc aaacccttct ctatcagatc gttgacgagt attacccggc attcgctgcg
      241 cttatggcag agcagggaaa ggaattgccg ggctatgtgc aacgggaatt tgaagaattt
      301 ctccaatgcg ggcggctgga gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc
      361 gagcacctgg tcgctttcag ctgtaagcgt cgcggtttct gcccgagctg tggggcgcgg
      421 cggatggccg aaagtgccgc cttgctggtt gatgaagtac tgcctgaaca acccatgcgt
      481 cagtgggtgt tgagcttccc gtttcagctg cgtttcctgt ttgccagccg gcccgagatc
      541 atggggtggg tgctgggcat cgtttaccgc gtcattgcca cgcacctggt caagaaagcg
      601 ggccataccc accaagtggc caagacgggc gcggtcaccc tgatccagcg ttttggatcg
      661 gcgctcaatc tgaatgttca cttccacatg ctgtttctcg acggtgtgta tgtcgagcaa
      721 tcccacggct cagcgcgttt ccgctgggtc aaggcgccga ccagcccaga gctcacccag
      781 ctgacgcaca ccatcgccca ccgggtgggt cgctatctgg aacggcaagg cctgctggaa
      841 cgggatgtcg aaaacagcta tctggcctcg gatgcggtgg atgacgaccc gatgacaccc
      901 ctgctggggc actcgatcac ttaccgtatc gctgtcggtt cacaggcggg gcgaaaggtg
      961 ttcactttgc aaactctgcc gaccagtggt gatccgttcg gtgacgggat tggcaaggta
     1021 gccgggtcca gcctgcacgc cggcgtggcg gccagggccg atgaacgcaa gaagctcgaa
     1081 cggctgtgcc ggtacatcag ccgcccggcg gtatccgaga agcggctgtc gttaacacga
     1141 ggcggcaacg tgcgctacca gctcaagacg ccgtaccggg acggcaccac gcacgtcatt
     1201 ttcgaaccat tggatttcat tgcaaggctg gccgccctgg taccgaagcc cagagtcaac
     1261 ctaacccgct tccacggggt gttcgcaccc aacagtcggc accgggcgtt ggtcacgccg
     1321 gcaaaacggg gcaggggcaa caaggtcagg gtggctgatg aaccggcaac accagcacaa
     1381 cggcgagcgt cgatgacatg ggcgcaacgg ctcaagcgtg ttttcaatat cgacatcgag
     1441 acctgcagcg gctgcggcgg cgccatgaaa gtcatcgcct gcattgaaga ccctatagtg
     1501 atcaagcaga tccttgatca cctgaagcac aaagccgaaa ccagcgggac cagggcgtta
     1561 cccgaaagcc gggcgccacc ggctgagctg ctcctgggtc tgtttgactg acgagcctga
     1621 aggccaacga taccaatcaa aatgctgcgt tcacagcgcc gcggcaggga tccgccgtgc
     1681 tggttgtcgg aaaaggagcc gctagtggga aagaggaggg taaattttca gcgttgctgg
     1741 ctccccgtca gccggattgg gttgcatcgc aggggtgtcg aaagagtcaa ctgcggtcca
     1801 aagctgttgg acttgggtga aaagggcgtt tattcttcct atacgtgccg cgttccaggc
     1861 ggcgactatg agggctatgt cgatgcccat gtgcgccggc tggaggcgct acgccgggcc
     1921 ggtatcgtcg agcggatcga cgccgaccaa tggcgcatcc ccaatgatct ggtcagccgt
     1981 gccgccgccc atgacgccgg ccgagacagt caggccagcg ttcgcgtcct ttccccggtc
     2041 gatctgaaca aacagatcgg atcggacggc gcgacctggc tggaccggcg gctgatccac
     2101 ggcgagacgg ccgaccttgc gccaaccggc ttcgggcaac aagtccgcga agccatggac
     2161 cagcgccgcg agcaccatat cgaacagggc gacgccaccc gcagccggga cagccgcgtc
     2221 ttctaccggc gcaaccttct cgccatcctg cgggagcgcg aggtagccgg cgtcggatcg
     2281 gatatggctt tgagtaaggg cctgccgttc cgcgccgcca cggacggcga gagcgtcagc
     2341 ggcaagttta ccggaaccgt gcatctatcg agcggcaagt tcgccgtggt cgagaaatcc
     2401 catgagttca cccttgtccc gtggcggccg atcatcgacc gccaactcgg ccgcgaggtt
     2461 atgggcatcg tgcagggcgg gtcggtgtcg tggcagttag ggcggcagag ggggctggaa
     2521 cgctgagtgc gcccatgccg cattgcgaag caaaagataa tcggataaaa tgtagcaatt
     2581 catattcgta agcgtggagt aatcagatgg gaaattccaa gtcagcagac aagtaagccg
     2641 caacaaccag tattgttgtt gcggcgctct gtaaggctag tctcatctga ttgctgacga
     2701 gcagacgtcg cccggtattc cttaatcgag ggttgattcg tcatgaccac cacacgcccc
     2761 gcgtgggcct atacgctgcc ggcagcactg ctgctgatgg ctcctttcga catcctcgct
     2821 tcactggcga tggatattta tctccctgtc gttccagcga tgcccgacat cctgaacacg
     2881 acgcccgcta tgatccaact cacgttgagc ctctatatgg tgatgctcgg cgtgggccag
     2941 gtgatttttg gtccgctctc agacagaatc gggcgacggc caattctact tgcgggcgca
     3001 acggctttcg tcattgcgtc tctgggagca gcttggtctt caactgcacc ggcctttgtc
     3061 gctttccgtc tacttcaagc agtgggcgcg tcggccatgc tggtggcgac gttcgcgacg
     3121 gttcgcgacg tttatgccaa ccgtcctgag ggtgtcgtca tctacggcct tttcagttcg
     3181 atgctggcgt tcgtgcctgc gctcggccct atcgccggaa cattgatcgg cgagttcttg
     3241 ggatggcagg cgatattcat tactttggct atactggcga tgctcgcact cctaaatgcg
     3301 ggtttcaggt ggcacgaaac ccgccctctg gatcaagtca agacgcgccg atctgtcttg
     3361 ccgatcttcg cgagtccggc tttttgggtt tacactgtcg gctttagcgc cggtatgggc
     3421 acctacttcg tcttcttctc gacggctccc cgtgtgctca taggccaagc ggaatattcc
     3481 gagatcggat tcagctttgc cttcgccact gtcgcgcttg taatgatcgt gacaacccgt
     3541 ttcgcgaagt cctttgtcgc cagatggggc atcgcaggat gcgtggcgcg tgggatggcg
     3601 ttgcttgttt gcggagcggt cctgttgggg atcggcgaac tttacggctc gccgtcattc
     3661 ctcaccttca tcctaccgat gtgggttgtc gcggtcggta ttgtcttcac ggtgtccgtt
     3721 accgcgaacg gcgctttggc agagttcgac gacatcgcgg gatcagcggt cgcgttctac
     3781 ttctgcgttc aaagcctgat agtcagcatt gtcgggacat tggcggtggc acttttaaac
     3841 ggtgacacag cgtggcccgt gatctgttac gccacggcga tggcggtact ggtttcgttg
     3901 gggctggtgc tccttcggct ccgtggggct gccaccgaga agtcgccagt cgtctaaccg
     3961 acgactggta gcaggcccgc tccgatgcgg cgcactaacc atcgaaacct cgtgaatgtc
     4021 ggtatcctgt ctggcaggat accgctcatt tcccttgttc agttcatcgc cgtcgccgag
     4081 catctgaatt ttcggcatgc ggccaaggca cttggtatca gccagtcgag cgtcagcgcg
     4141 cgtgtgaaag cgctggagga taaccttggt gtcctgctat ttgagcgcca tgcgcggggc
     4201 gttcggctaa cagacgcagg caggcacttc atggagcgtg tcacggcggg tgtcgatcaa
     4261 ctcgatcacg cagtgaagac cgcggagtga
//
